Composite

Part:BBa_K3009004:Design

Designed by: Carolin Ruckes   Group: iGEM19_Freiburg   (2019-10-15)


sfGFP with amber inside chrom.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1107
    Illegal EcoRI site found at 4055
    Illegal XbaI site found at 3608
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1107
    Illegal EcoRI site found at 4055
    Illegal NheI site found at 1689
    Illegal NheI site found at 3572
    Illegal NheI site found at 3595
    Illegal NheI site found at 4049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1107
    Illegal EcoRI site found at 4055
    Illegal BamHI site found at 3988
    Illegal XhoI site found at 4509
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1107
    Illegal EcoRI site found at 4055
    Illegal XbaI site found at 3608
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1107
    Illegal EcoRI site found at 4055
    Illegal XbaI site found at 3608
    Illegal NgoMIV site found at 2622
    Illegal NgoMIV site found at 5917
    Illegal AgeI site found at 503
    Illegal AgeI site found at 1775
    Illegal AgeI site found at 2099
    Illegal AgeI site found at 3120
    Illegal AgeI site found at 3823
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4981
    Illegal BsaI site found at 5605
    Illegal SapI site found at 3805


Design Notes

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Source

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References